10X PBS Buffer pH 7.2 with Azide

Catalog No. ABIN925551

Supplier Product No.: mb-011

Quick Overview for 10X PBS Buffer pH 7.2 with Azide (ABIN925551)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Supplier Product No.: mb-011

Supplier: Rockland

Purpose: 10X PBS Buffer pH 7.2 with Azide

Purification: 10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. 10X PBS pH 7.2 with Azide was tested on trypticase

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:10

Application Note: Phosphate Buffered Saline 10X pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1 % (w/v) Sodium Azide) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final worki

Western Blot Dilution: 1:10

ELISA Dilution: 1:10

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 X

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature

Storage: RT,4 °C

Storage Comment: Store container at room temperature (18° to 26° C) prior to opening. If desired, 10X PBS pH 7.2 with Azide may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature ( Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Background:

Synonyms: Phosphate Buffered Saline

Background: Phosphate Buffered Saline (PBS) is a regularly used buffer in biological research. The phosphate ions help maintain a constant pH around neutral. The osmolarity of solution typically matches that of the human body (isotonic solution). PBS can act as a